Methods and apparatus are provided for assessing endothelial function in a mammal. In certain embodiments the methods involve using a cuff to apply pressure to an artery in a subject to determine a plurality of baseline values for a parameter related to endothelial function as a function of applied pressure (Pm)； b) applying a stimulus to the subject； and applying external pressure Pm to the artery to determine a plurality of stimulus-effected values for the parameter related to endothelial function as a function of applied pressure (Pm)； where the baseline values are determined from measurements made when said mammal is not substantially effected by said stimulus and differences in said baseline values and said stimulus-effected values provide a measure of endothelial function in said mammal.
