Methods for the production of a cosmetic composition from a hatching fluid from Salmonidae eggs and uses thereof to improve the cosmetic appearance of the skin
A method to reduce or prevent the cosmetic appearance or predominance of wrinkles, fine lines, hyperpigmentation, laxity, dry skin, scale formation and / or loss of transepidermal water on the skin of a mammalian animal, which comprises administering a cosmetic composition to said animal, wherein said composition is obtained or can be obtained with a method comprising at least the steps of: a) suspending Salmonidae eggs in a minimum volume of water, preferably salmon eggs； b) induce rapid, synchronized hatching of said eggs, preferably so that the hatching is completed in less than 2 hours for more than 95% of the embryos； c) filter the hatched eggs to obtain hatching fluid； and d) filtering the hatching fluid to obtain the composition, wherein the step of filtering the hatching fluid comprises at least the steps of: (i) filtering the hatching fluid using a filter with a pore size of at least 5 μm, and collect the filtrate； (ii) filter the filtrate from step (i) using a filter with a pore size of 0.30-0.60 µm, and collect the filtrate； (iii) exchanging the water in the filtrate of step (ii) with a pharmaceutically acceptable buffer by performing diafiltration using a filter with an exclusion size of less than 15 kDa； (iv) optionally repeat the diafiltration stage of stage (iii)； (v) optionally diluting the solution of step (iii) or (iv) so that the solution has an enzymatic activity of 3000-5000 mU / l, measured by the solution's ability to cleave the Factor Xa chromogenic substrate ( CH3OCO-D-CHA-Gly-Arg-pNA-AcOH)； (vi) filter the solution from step (iii), (iv), or (v) using a filter with a pore size of 0.15-0.30 μm, and collect the filtrate； and (vii) preparing said cosmetic composition from the filtrate of step (vi).Un método para reducir o prevenir el aspecto cosmético o predominio de arrugas, líneas finas, hiperpigmentación, laxitud, piel seca, formación de escamas y/o pérdida de agua transepidérmica en la piel de un animal mamífero, que comprende administrar u
