The present invention provides a method for producing an exosome that transfers an active substance specifically to a target and the exosome produced by the same； a method for delivering the active substance to the target tissue using the exosome； a pharmaceutical composition for delivery of the active substance comprising the exosome as an active ingredient； and a composition for preparing the exosome comprising an expression vector wherein the target peptide is inserted into an extracellular portion of a transmembrane protein.
