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Uk-2 biosynthetic genes and method for improving uk-2 productivity using the same
专利权人:
Meiji Seika Pharma Co. Ltd.
发明人:
Kobayashi Koei,Sumida Naomi,Yanai Koji
申请号:
NZ70375613
公开号:
NZ703756A
申请日:
2013.07.08
申请国别(地区):
NZ
年份:
2017
代理人:
摘要:
To provide a production method capable of mass production of UK-2 at low cost, the genomic DNA of Streptoverticillium sp. 3-7, which produces UK-2, was analyzed to identify a region expected to be a UK-2 biosynthetic gene cluster. Moreover, by colony hybridization, DNAs in the region were successfully isolated. Further, the DNAs were used to prepare a strain in which the genes present in the region were disrupted. The strain was found not to produce UK-2. It was verified that the genomic region was the UK-2 biosynthetic gene cluster. Furthermore, Streptoverticillium sp. 3-7 was transformed by introduction of a vector in which the isolated UK-2 biosynthetic gene cluster was inserted. It was also found out that the UK-2 productivity by the transformant was improved about 10 to 60 times or more in comparison with that of the parental strain. Moreover, it was revealed that 2 copies of the UK-2 biosynthetic gene cluster were present per cell in these transformants, respectively.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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