The invention provides a system and method for using OCT to determine hydration and blood flow variation adjusted glucose measurement in a target of interest. For some target, including a target of a living human, the invention provides for the measurement of tissue components, for determination of hydration level and blood flow variations of target, for non-invasive determination of glucose in target, and for hydration-adjusted and blood flow compensated glucose measurement in target.In one embodiment, both the tissue components and glucose level are measured by OCT, and tables or indexes of hydration are used to determine hydration adjustment.In alternate embodiments, the hydration index may be partially or fully “individualized,” created from data collected from monitoring an individual or groups of individuals with pre-selected characteristics (ex. same gender, weight, age, ethnicity, diabetes stage, species, etc.). Additional embodiments include blood flow variation monitoring and blood flow compensated glucose measurement.
