Disclosed i s a method for natural species rejuvenation of artificially bred strains, comprising the following stes 1) infecting cordyceps host larvae with cordyceps strains artificially bred for more than 2 generations, screening the contaminated larvae which are firstly rejuvenated, and placing the larvae in a rejuvenation base after continuously culturing for 1 to 2 stages 2) managing the transition periods so that the contaminated larvae adapt to the wild environment 3) managing the specie nature exercise period of the bombyx insects 4) rejuvenation of natural species of sexual spores and mycodermas 5) collecting cordyceps with mature sexual spores, and transplanting to a sterilized culture medium to continuously culture after washing the insect-strain complexes 6) after-ripening of the cordyceps and 7) harvesting cordyceps strain materials. The method provides a strain guarantee for large-scale sustainable production of cordyceps.