Bruno Jean-Marie Florin;Bernard Masseret;Caroline Denise Monique Vachet
发明人:
Bruno Jean-Marie Florin,Bernard Masseret,Caroline Denise Monique Vachet
申请号：
US12745753
公开号：
US08921087B2
申请日:
2008.12.01
申请国别(地区):
US
年份:
2014
代理人:
摘要：
A process for the propagation of Theobroma cacao L. by somatic embryogenesis using explant material which is subjected to a) primary embryogenesis in the dark in a solid culture medium causing induction and expression to produce primary embryos, b) secondary embryogenesis i) in which the primary embryos are treated in the dark in a solid or liquid culture medium to produce and multiply embryogenic callus followed by ii) treatment of the embryogenic callus in the dark in a suitable liquid culture medium causing expression of the embryogenic callus to produce further new secondary embryos, c) pre-germination of the secondary embryos in a Petri dish on a solid medium, or in a bioreactor in a liquid medium, into pre-germinated secondary embryos at the cotyledonary stage, d) ex-vitro germination of the pre-germinated secondary embryos at the cotyledonary stage by sowing directly on a culture substrate in the greenhouse to produce the plantlets, and e) development of the plantlets.
