A sterilized connection apparatus 4 includes a sealing unit 9 which forms a decontamination space S1 between an isolator 2 provided with a first cover body 11 and an incubator 3 provided with a second cover body 21, a decontamination medium supplying unit 10 which supplies a decontamination medium to the decontamination space S1 via a supply pipe line 41, and a discharge pipe line 43 which discharges the decontamination medium of the decontamination space S1.Further, a cover body connecting unit 8 which couples the first cover body 11 and the second cover body 21 to form a circular decontamination space S1 in the periphery of these bodies is provided, and a shielding member 36 which blocks circulation of the decontamination medium is provided on the inside of the circular decontamination space S1 to divide the circular decontamination space S1 to form a long and thin pipe line-shaped decontamination space S1. In addition, the supply pipe line 41 is connected to both ends of the long and thin pipe line-shaped decontamination space S1, and the discharge pipe line 43 is connected between these ends.Since the decontamination space is formed as a circular decontamination space of a small capacity surrounding the cover bodies, decontamination can be efficiently performed.
