It is described a disinfecting composition containing glycolic acid, at least one C 1-C 6 alcohol and at least one quaternary ammonium compound selected from the group consisting of C8-C16-alkyl benzyl dimethyl ammonium chloride, C 8-C 6-alkyldimetylamoniumchlorid and di(C 8-C 16-alkyl) dimethyl ammonium chloride. Further is described a method of disinfecting and its use.Opisuje sa dezinfekčný prípravok obsahujúci kyselinu glykolovú, aspoň jeden C1-C6 alkohol a aspoň jednu kvartérnu amóniovú zlúčeninu vybranú zo skupiny zahŕňajúcej C8-C16-alkylbenzyl dimetylamóniumchlorid, C8-C6-alkyldimetylamóniumchlorid a di(C8-C16-alkyl)dimetylamónium chlorid. Ďalej sa opisuje spôsob dezinfekcie a jeho použitie.