A puncture apparatus includes a puncture needle for puncturing living body tissue of a living body, and a vaginal-insertion assembly positionable in a vagina of the living body. The vaginal-insertion assembly includes a vaginal-insertion member and an inserter connected to the vaginal-insertion member. The inserter is connected to the vaginal-insertion member so that the distal-most end portion of the inserter is located distally beyond the distal-most end portion of the vaginal-insertion member. The size of distal-most end portion of the inserter is less than the size of the distal-most end portion of the vaginal-insertion member. The vaginal-insertion member and the distal-most end portion of the inserter are movable relative to each other to reduce the distance between the distal-most end of the inserter and the distal-most end of the vaginal-insertion member after inserting the vaginal insertion assembly into the vagina.