The invention relates to a sterilization system with automatic control of ozone supply, which includes: a controllable ozone unit provided with an oxygen machine that outputs oxygen; a controller for controlling a driver to drive a single-phase sine wave pulse width modulation high frequency inverter to output a high voltage, wherein the high voltage electric shock a ceramic plate to ionize oxygen to generate an ozone; a water quality detection unit for detecting an index parameter of cooling water, comparing the detected index parameter with a water quality standard value, and outputting a water quality feedback signal; and a water quality feedback control circuit signally connected to the controllable ozone unit and the water quality detection unit independently, wherein the water quality feedback control circuit receives the water quality feedback signal to start or stop the controllable ozone unit to produce ozone, and to further fine-tune the ozone concentration, so that the ozone concentration can be monitored precisely without staffing.本發明係關於一種自動控制臭氧供應量的消毒系統,包含:一可控式臭氧單元設有氧氣機輸出氧氣,一控制器控制驅動器以驅動一單相式弦波脈衝寬度調變高頻逆變器輸出一高壓電,該高壓電電擊陶瓷平板,使氧氣電離產生一臭氧;一水質檢測單元,檢測一冷卻水中的一指標參數與一水質標準值進行比對,並於檢測後輸出一水質回授訊號;一水質回授控制電路,分別訊號連接該可控式臭氧單元及該水質檢測單元,該水質回授控制電路接收該水質回授訊號,以供啟動或關閉可控式臭氧單元產生臭氧,並進一步微調臭氧濃度,藉以不需要人力即可精準監控。