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DÉTERMINATION DE LYMPHOCYTES T ET DE LYMPHOCYTES B SPÉCIFIQUES AUX ANTIGÈNES
专利权人:
Adaptive Biotechnologies Corp.
发明人:
KLINGER, Mark,FAHAM, Malek
申请号:
EP20157220
公开号:
EP3674415A1
申请日:
2015.04.01
申请国别(地区):
EP
年份:
2020
代理人:
摘要:
The invention is directed to methods for determining antigen-specific lymphocytes, including T cells or B cells. In some embodiments, methods of the invention may be implemented by the steps of reacting under interaction conditions one or more antigens with lymphocytes in a plurality of subsets of a tissue sample, such as peripheral blood; sorting antigen-interacting lymphocytes from other lymphocytes; separately sequencing for each subset recombined nucleic acid encoding a segment of a TCR chain or BCR chain from a sample of T cells or B cells, respectively, prior to exposure to antigen and from a sample of T cells or B cells isolated based on their interaction with antigen, respectively, thereby forming a clonotype profile for the former sample and the latter sample for each subset; and identifying as antigen-specific lymphocytes those lymphocytes associated with a clonotype whose frequency increases in the latter sample relative to its frequency in the former sample.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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