The invention particularly relates to a citicoline pharmaceutical composition for injection, its preparation method and application thereof. The choline injection raw material and auxiliary materials provided by the invention include: citicoline, activated carbon, traditional Chinese medicine extract and water for injection; the preparation method of citicoline injection is as follows: (1) preparation and weighing according to prescription Amount of various raw materials; (2) first add appropriate amount of water for injection into the concentrated tank, and then add the corresponding raw materials and stir in order, until they are completely dissolved, add activated carbon and stir, then adjust the pH value, and finally add water for injection to the configured amount; (4) test and fill the above liquid Packed and sterilized. The note prepared by the preparation method of the invention. The ejaculation has a good therapeutic effect on hemiplegia caused by stroke and obstacles of limb function, as well as other functional and conscious disturbances caused by acute injury of the central nervous system.