Ahmed E. Yousef,Yaoqi Guo,En Huang,Chunhua Yuan,Liwen Zhang
申请号：
US13705936
公开号：
US09017692B2
申请日:
2012.12.05
申请国别(地区):
US
年份:
2015
代理人:
摘要：
Provided herein is a biologically pure culture of Paenibacillus thiaminolyticus, identified as OSY-SE, as well as an antimicrobial agent isolated and/or purified from the culture having any one of SEQ ID NOs:1-3 and 64-66. The disclosure also provides compositions and articles of manufacture comprising an antimicrobial agent and/or the bacterial cell of Paenibacillus thiaminolyticus, identified as OSY-SE. Further provided are methods of use, including methods of affecting microbial activity, methods of inhibiting growth and/or proliferation of a microbe, methods of treating a condition or disease associated with the presence of a microbe, and methods of treating a microbial infection in a subject comprising contacting a microbial cell with at least one active agent of SEQ ID NOs:1-3 and 64-66 and/or the bacterial cell Paenibacillus thiaminolyticus, identified as OSY-SE. The disclosure also provides the biosynthetic machinery (e.g., utilizing a NRPS mechanism) including isolated proteins, isolated polynucleotides, vectors, and host cells for production of the antimicrobials described herein.
