Acellular collagenous tissue and a processing method of an artificial valve comprising the acellular collagenous tissue. The method comprises: impregnating an acellular collagenous tissue in a preservation solution comprising ionic liquid until the size of the acellular collagenous tissue remains stable in a non-liquid environment. Also provided is a preservation method, comprising: impregnating the acellular collagenous tissue or the artificial valve comprising the acellular collagenous tissue in the preservation solution comprising ionic liquid until the size of the acellular collagenous tissue remains stable in the non-liquid environment; after the acellular collagenous tissue or the artificial valve comprising the acellular collagenous tissue is obtained, removing an extra preservation solution attached to a surface thereof, and performing packaging. Also provided are the acellular collagenous tissue and the artificial valve comprising the acellular collagenous tissue processed by using the method. The method can effectively prevent a biological material from being damaged in the non-liquid environment and is beneficial to subsequent processing and sterilization processing implemented on the biological material.L'invention concerne un tissu collagénique acellulaire et un procédé de traitement d'une valvule artificielle comprenant le tissu collagénique acellulaire. Le procédé consiste à imprégner un tissu collagénique acellulaire dans une solution de conservation comprenant un liquide ionique jusqu'à ce que la taille du tissu collagénique acellulaire reste stable dans un environnement non liquide. L'invention concerne également un procédé de conservation, qui consiste à : imprégner le tissu collagénique acellulaire ou la valvule artificielle comprenant le tissu collagénique acellulaire dans la solution de conservation comprenant un liquide ionique jusqu'à ce que la taille du tissu collagénique acellulaire reste stable dans l'environnement non liquide; après l'obt