A method of ultrasound nonlinear imaging with Golay code excitation includes transmitting a first and a second Golay code signal wave which are orthogonal by each other making the second Golay code signal wave be subtracted from the first Golay code signal wave to eliminate a first and a second noise interference wave to generate a third Golay code signal wave processing a first and a second compression filter process to the third Golay code signal wave to generate a first and a second compress code signal wave taking the difference between the first compress code signal wave and the second compress code signal wave to generate a third compress code signal wave which includes at least two second-order harmonic waves processing ultrasound nonlinear imaging by using the multi-frequency harmonic component waves to generate an ultrasound image.一種應用於超音波非線性成像之格雷編碼成像方法,其包含發射彼此正交之第一與第二格雷編碼信號波形;使第一格雷編碼信號波形減去第二格雷編碼信號波形,藉以消除未正確編碼干擾,據以產生一第三格雷編碼信號波形;對第三格雷編碼信號波形分別進行一第一與第二壓縮濾波處理,藉以產生第一與第二壓縮編碼信號波形;使第一壓縮編碼信號波形減去第二壓縮編碼信號波形,藉以產生一包含至少二壓縮二階諧波波形之第三壓縮編碼信號波形以供超音波非線性成像。