The injection device (100) has an actuating device (20) with an actuating element (21) that forms an abutment for a piston device (53) of the injection syringe (50) on the guide device (10) in such a manner that during the withdrawal movement. The piston device executes a relative movement counter to the injection needle (51) and a syringe body (52). Independent claims are also included for the following: (1) a method for injection of injection substance in an organism (2) an injection depot, which is arranged in a biological fabric (3) an autologous myoblast, particularly autologous serum for the treatment of fecal incontinence (4) a method for the production of a drug for the treatment of fecal incontinence.