An imaging apparatus includes an imaging unit and a signal processing unit. The imaging unit generates a first image signal on the basis of visible light from an object and generates a second image signal on the basis of excitation light and fluorescence from the object. The signal processing unit generates a fluorescence image signal corresponding to the fluorescence on the basis of the first image signal and the second image signal. The signal processing unit determines a target area of the object on the basis of the first image signal. The signal processing unit determines a fluorescence area on the basis of the second image signal corresponding to the target area, and the fluorescence area generates the fluorescence in the object. The signal processing unit performs an emphasis process of the second image signal corresponding to the fluorescence area.
