The presently disclosed subject matter provides systems and methods forproducing a three-dimensional model of ahuman cervix. A microdevice is provided for culturing human cervical cells.The microdevice can include an upper microchannelincluding live ectocervical epithelial cells. The microdevice can include alower microchannel including a first parallel lane and asecond parallel lane including stromal media. The first and the secondparallel lanes can be lined with live vascular endothelial cells.The lower microchannel can include a third parallel lane including uterinefibroblasts and live smooth muscle cells embedded inhydrogel. The first, second, and third lanes of the lower microchannel can beseparated by protrusion structures. The third parallel lanecan be positioned in the lower microchannel in between the first and thesecond parallel lanes. The microdevice can further include aporous membrane positioned in between the upper microchannel and the lowermicrochannel.
