The present invention relates toLigustrumLucidumAiton), HwanggiAstragalusMembranaceusBunge,CitrusUnshiuMarkovich,AngelicaDahurica The present invention relates to a cosmetic composition for skin moisturizing improvement, a skin external preparation for skin moisturization, and a pharmaceutical composition for prevention and treatment of skin diseases. More specifically, the present invention relates to a cosmetic composition for improving skin moisturization, comprising a mixture of western blot and luciferase Through the luciferase reporter assay, it was confirmed that protein expression and promoter activity of the factors related to keratinocyte differentiation were increased, and it was confirmed that there was a significant effect through experiments of transepidermal water loss (TWEL) , It has been confirmed that the mixed extract of the present invention can be effectively used as an effective ingredient of a cosmetic composition for skin moisturizing, a skin external preparation for skin moisturizing, and a pharmaceutical composition for skin disease prevention and treatment.본 발명은 여정자(Ligustrumlucidum Aiton), 황기(Astragalusmembranaceus Bunge), 귤피(Citrus unshiu Markovich), 백지(Angelica dahurica root) 혼합 추출물을 함유하는 피부 보습 개선용 화장료 조성물, 피부 보습용 피부 외용제 및 피부 질환 예방 및 치료용 약학적 조성물에 관한 것으로서, 구체적으로 본 발명의 혼합 추출물은 웨스턴 블롯(western blot) 및 루시퍼레이즈 리포터 분석(luciferase reporter assay)을 통해서 각질세포 분화에 관련된 인자들의 단백질 발현 및 프로모터(promoter)활성이 증가 됨을 확인하고, 표피 수분 손실(transepidermal water loss, TWEL) 실험을 통해 유의한 효과가 있음을 확인함으로써, 본 발명의 혼합 추출물을 피부 보습용 화장료 조성물, 피부 보습용 피부 외용제 및 피부 질환 예방 및 치료용 약학적 조성물의 유효성분으로 유용하게 사용될 수 있음을 확인하였다.
