A process for the preparation of alkaliphilic and thermophilic xylanase which comprises cultivating Pseudomonas stutzeri such as herein described, in a nutrient medium such as herein described, containing assimilable nitrogen source, supplemented by conventional trace elements such as herein described, and a source of xylan in a known manner at a pH ≥ 7, separating cells from supernatant by conventional methods and recovering alkaliphilic and thermophilic xylanase from cell free supernatant by conventional precipitation method followed by conventional partial purification using dialysis technique.
