Methods of and apparatuses for equine plasma collection are provided. Modified plasmapheresis instruments and modified disposable plasmapheresis tubing are introduced. By using the modified procedures and instruments, a high-speed, high volume, continuous-flow process of automated equine plasmapheresis is developed. The modified closed systems remove whole blood from the donor horses, separate plasma from the cellular components of blood, and simultaneously returning concentrated cells back to the donor horses.
