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TETRANEKTIN-apolipoprotein A-I, containing its lipid particles AND ITS APPLICATION
专利权人:
Ф. ХОФФМАНН-ЛЯ РОШ АГ (CH)
发明人:
БАДЕР Мартин (DE),БЕНЕР Моника (DE),ФИНГЕРЛЕ Йюрген (DE),КОНЕРТ Ульрих (DE),МАРИ Жан-Люк (FR),МОЛЬ Зильке (CH)
申请号:
RU2013112562/15
公开号:
RU2013112562A
申请日:
2011.08.25
申请国别(地区):
RU
年份:
2014
代理人:
摘要:
1. A lipid particle including apolipoprotein AI or a variant thereof, i-1-palmitoyl-2-oleyl-phosphatidyl choline and 1,2-dipalmitoyl-phosphatidyl choline, and i-detergent, where the molar ratio of 1-palmitoyl-2-oleyl- phosphatidyl choline to 1,2-dipalmitoyl-phosphatidyl choline is from 99: 1 to 25: 75.2. A lipid particle according to claim 1, characterized in that the apolipoprotein A-I or a variant thereof is a multimer comprising three monomers. The lipid particle according to claim 1, wherein the apolipoprotein AI is tetranectin-apolipoprotein AI having the amino acid sequence of SEQ ID NO: 01, or SEQ ID NO: 02, or SEQ ID NO: 06, or SEQ ID NO: 66, or SEQ ID NO: 67.4. A lipid particle according to claim 1, characterized in that the amino acid sequence of apolipoprotein A-I has one or more conservative amino acid modifications. The lipid particle according to claim 1, characterized in that the amino acid sequence of apolipoprotein AI is at least 70% homologous to the amino acid sequence of SEQ ID NO: 01, or SEQ ID NO: 02, or SEQ ID NO: 06, or SEQ ID NO: 66 , or SEQ ID NO: 67.6. The lipid particle according to claim 1, characterized in that it binds to a receptor selected from the group consisting of cubilin, class B scavenger receptor, type 1 (SR-BI), ATP-binding cassette 1 (ABCA-1), lecithin-cholesterol acyltransferase (LHAT), cholesterol ester transfer protein (CETP) or phospholipid transfer protein (PLTP). 7. The lipid particle according to claim 1, characterized in that the number of phospholipid molecules per apolipoprotein monomer in the lipid particle is from 40 to 120.8. The lipid particle according to claim 1, characterized in that the number of phospholipid molecules per apolipoprotein monomer is from 50 to 110.9. The lipid particle according to claim 1, characterized1. Липидная частица, включающая- аполипопротеин A-I или его вариант, и- 1-пальмитоил-2-олеил-фосфатидил холин и 1,2-дипальмитоил-фосфатидил холин, и- детергент,где молярное соотношение 1-паль
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