The present invention relates to methods of eliminating, reducing or preventing bacterial biofilms by means of a fusion protein comprising an endolysin, an autolysin or a bacteriocin to which a peptide with membrane or LPS disrupting activity is fused. Further, the present invention relates to fusion proteins for use as a medicament, in particular for the treatment or prevention of Gram-negative and/or Gram-positive bacterial infections associated with bacterial biofilm, as diagnostic means, disinfectant or as cosmetic substance. The present invention also relates to the removal or reduction or prevention of Gram-negative and/or Gram-positive bacterial contamination associated with bacterial biofilm of foodstuff, of food processing equipment, of food processing plants, of surfaces coming into contact with foodstuff, of medical devices, of surfaces in hospitals and surgeries. Furthermore, the present invention relates to the use of said fusion protein as a diagnostic means in medicinal, food or feed or environmental diagnostic associated with bacterial biofilm.本發明涉及消除、減少或阻止細菌生物膜的方法，其依靠包含與具有膜或LPS破壞活性的肽融合的內溶素、自溶素或細菌素的融合蛋白來進行。此外，本發明涉及作為藥物(特別是用於治療或預防與細菌生物膜有關的革蘭氏陰性和/或革蘭氏陽性細菌感染)、作為診斷手段、消毒劑或作為化妝用物質使用的融合蛋白。本發明還涉及除去或減少或阻止食品、食物加工設備、食物加工廠、與食品接觸的表面、醫學裝置、醫院和診所的表面的與細菌生物膜有關的革蘭氏陰性和/或革蘭氏陽性細菌污染。此外，本發明涉及所述融合蛋白作為診斷手段在與細菌生物膜有關的醫學、食物或飼料或環境診斷中的用途。
