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MODIFIED EPITOPES FOR STRENGTHENING CD4 + T-CELL RESPONSES
专利权人:
КАТОЛИКЕ УНИВЕРСИТЕЙТ ЛЕВЕН (BE);ЛАЙФ САЙЕНСИЗ РИСЕРЧ ПАРТНЕРЗ ВЗВ (BE)
发明人:
СЭН-РЕМИ Жан-Мари (BE)
申请号:
RU2014135406
公开号:
RU2014135406A
申请日:
2013.01.30
申请国别(地区):
RU
年份:
2016
代理人:
摘要:
1. A method for producing a population of activated non-cytolytic CD4 + T cells in vitro, where the method includes the step of introducing into the peripheral blood cell population an isolated immunogenic peptide from 9 to 50 amino acids long, where the peptide contains: a) a T-cell epitope of 8 or 9 amino acids antigenic protein for MHC class II and b) a sequence of 1 to 6 amino acids from the n-terminal and / or c-terminal side of the sequence of the epitope of 8 or 9 amino acids from a) containing a reducing cysteine residue, where the sequence is from 1 to 6 amino acids does not contain a motif sequence with redox activity of C-xx- [CST] or [CST] -xx-C.2. The method of claim 1, wherein the peptide is an artificial peptide. The method of claim 1, wherein the sequence defined in part b) contains only one cysteine. The method of claim 1, wherein the immunogenic peptide has a length of from 9 to 20 amino acids. The method of claim 1, wherein said cysteine residue is localized directly from the N- or C-terminus of an 8 or 9 amino acid epitope, without amino acids between the sequence of said epitope and said cysteine residue. The method of claim 1, wherein said T cell epitope is a T cell epitope of an infectious agent. The method of claim 1, wherein said T-cell epitope is a T-cell epitope of its own antigen, allergen, allofactor, or allograft antigen. The method of claim 1, wherein said T cell epitope is a T cell epitope specific or preferred for a tumor. An isolated immunogenic peptide with a length of 9 to 30 amino acids capable of activating non-cytolytic CD4 + T cells and containing: a) a T-cell epitope of 8 or 9 amino acids antigenically1. Способ получения популяции активированных не цитолитических CD4+ Т-клеток in vitro, где способ включает стадию введения в популяцию клеток периферической крови выделенного иммуногенного пептида длиной от 9 до 50 аминокислот, где пептид содержит:a) Т-клеточный эпитоп из 8 или 9 аминокислот антигенного белка для МНС класса II и
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