SHEN CHANG ZHE,신장철,WON, HA YOUNG,원하영,KIM, SEUNG JU,김승주,LEE, JI YEON,이지연,SHIN, YEON HEE,신연희,LEE, SEON YOUNG,이선영,PARK, JUNG KEUN,박정근,KIM, EUI KYUNG,김의경,KIM, CHOUL GOO,김철구
申请号：
KR1020130065203
公开号：
KR1013645060000B1
申请日:
2013.06.07
申请国别(地区):
KR
年份:
2014
代理人:
摘要：
The present invention relates to a method for producing an isolated and purified bee venom without allergenic ingredients to produce the isolated and purified bee venom having a medicinal effect while basically preventing allergy induction by reducing or removing the allergenic ingredients in the bee venom using ultrafiltration. The method comprises: a first step for preparing the purified bee venom powder； a second step for making a bee venom solution by mixing the purified bee venom powder and distilled water； a third step for removing the allergenic ingredients contained in the bee venom solution by filtering the bee venom solution through an ultrafiltration membrane with cut-off size of 10 kDa； and a fourth step for obtaining the isolated and purified bee venom without the allergenic ingredients. At the second step, 1000-1500 ml of the distilled water is mixed with 1 g of the purified bee venom powder. At the third step, the allergenic ingredients removed through the ultrafiltration membrane are phospholipase A2 and hyaluronidase. The isolated and purified bee venom obtained at the fourth step contains at least 4% of apamin and at least 50% of melittin. [Reference numerals] (AA) START； (BB) END； (S11) Preparing purified bee venom powder； (S12) Making a bee venom solution by mixing the purified bee venom powder and distilled water； (S13) Removing the allergenic ingredients contained in the bee venom solution by filtering the bee venom solution through an ultrafiltration membrane with cut-off size of 10 kDa； (S14) Obtaining the isolated and purified bee venom without the allergenic ingredients본 발명은 한외여과방법으로 봉독 중 알러지 유발성분을 감축 또는 제거하여 약리 효과를 유지하면서도 알러지유발을 근본적으로 차단한 분리정제봉독을 제조할 수 있는 알러지성분이 분리된 분리정제봉독 제조방법에 관한 것으로, 정제봉독 분말을 준비하는 제 1단계, 상기 정제봉독 분말을 증류수와 혼합하여 봉독용액을 제조하는 제 2단계, 상기 봉독용액을 컷오프(cut-off) 사이즈가 10kDa(kilo-Dalton)인 한외여과 막(ultrafiltration membrane)으로 여과하여 상기 봉독용액에 포함된 알러지(allergy)성분을 제거하는 제 3단계 및 상기 알러지성분이 제거된 분리정제봉독을 수득하는 제 4단계를 포함하고, 상기 제 2단계에서, 상기 정제봉독 분말과 혼합되
