FEDERALNOE GOSUDARSTVENNOE BJUDZHETNOE UCHREZHDENIE "FEDERALNYJ NAUCHNO-ISSLEDOVATELSKIJ TSENTR EPIDEMIOLOGII I MIKROBIOLOGII IMENI POCHETNOGO AKADEMIKA N.F. GAMALEI" MINISTERSTVA ZDRAVOOKHRANENIJA RO
发明人:
GUDOV VLADIMIR PETROVICH,Гудов Владимир Петрович,SUBBOTINA MARINA EVGENEVNA,Субботина Марина Евгеньевна,GRA OLGA ALEKSEEVNA,Гра Ольга Алексеевна,GROMOV ALEKSANDR VIKTOROVICH,Громов Александр Викторови
申请号：
RU2015134903/05
公开号：
RU0002582702C1
申请日:
2015.08.19
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
FIELD: chemistry medicine.SUBSTANCE: invention relates to a method for preparing a composition based on modified sodium hyaluronate and use thereof in various fields of medicine, veterinary medicine and cosmetology. Method of preparing a composition based on modified copolymer of sodium hyaluronate and heparin comprises forming transverse covalent bonds between hydroxyl groups residues of D-glucuronic acid and D-N-acetylglucosamine included into composition of sodium hyaluronate, and residues of α-D-glucosamine and uronic acid related to heparin, by introducing active cross-linking bifunctional agent - (polyethylene glycol) diglycidyl ether (PEGDE) in an alkaline medium. After chemical copolymerisation, reaction with cross-linking agent is stopped. Obtained reaction mixture is neutralised to pH 7.0, then subjected to tangential filtration using a membrane with cut-off threshold 0.22 mcm, concentrated and stirred until complete homogenisation. Starting material used is animal-derived sodium hyaluronate of pharmacopoeial purity, subjected to tangential ultrafiltration to remove low-molecular fragments capable of triggering inflammatory response.EFFECT: biocompatibility, biodegradability and non-immunogenicity of modified compositions based on sodium hyaluronate allows use thereof in various fields of medicine and veterinary medicine, including surgery and arthroplasty, orthopedics, traumatology, ophthalmology, dermatology, aesthetic medicine and cosmetology.1 cl, 4 dwg, 10 exИзобретение относится к области химико-фармацевтической промышленности, биотехнологии и медицины, а именно к способу получения композиции на основе модифицированного гиалуроната натрия и ее применению в различных областях медицины, ветеринарии и косметологии. В способе получения композиции на основе модифицированного сополимера гиалуроната натрия и гепарина, заключающемся в создании поперечных ковалентных связей между гидроксильными группами остатков D-глюкуроновой кислоты и D-N-ацетилглюкозамина,
