Organ and tissue preservation formulations with improved stability shelf life are provided by separating the formulation into a first solution having a pH of at least 7 and a second solution having a pH of less than 7. The first solution includes components with improved stability when stored at a pH of 7 or above, and the second solution includes components with improved stability when stored at a pH below 7. The first solution includes water, a balanced salt solution, a sugar, adenosine, orotic acid, malic acid, L-carnitine, and insulin. The second solution includes water, an antioxidant such as ascorbic acid, a cellular reducing agent such as reduced glutathione, L-citrulline, creatine monohydrate, L-carnosine, and L-arginine. During use, the first and second solutions are mixed to form a final formulation that can be used at a physiological pH to preserve the function of the tissue or organ.本發明藉由將調配物分離成具有pH至少7之第一溶液及具有pH小於7之第二溶液而提供具有改進安定性儲存壽命之器官及組織保存調配物。該第一溶液包括在pH 7或以上儲存時具有改進安定性的組分,及該第二溶液包括在pH低於7儲存時具有改進安定性的組分。該第一溶液包括水、平衡鹽溶液、糖、腺苷、乳清酸、蘋果酸、L-肉鹼、及胰島素。該第二溶液包括水、抗氧化劑(諸如抗壞血酸)、細胞還原劑(諸如還原之麩胱甘肽)、L-瓜胺酸、肌酸單水合物、L-肌肽、及L-精胺酸。在使用期間,混合第一及第二溶液以形成最終調配物,其可在生理pH用於保存組織或器官之功能。22‧‧‧第一容器24‧‧‧第二容器26‧‧‧氧吸收劑28‧‧‧小袋
