Claim 1: a process of Manufacturing a composition inmunoterap\u00e9utica Custom to give a guy who has a disease or condition, such inmunoterap\u00e9utica Personalized composition comprises a Strain of Listeria attenuated recombinantWhere such a Strain of Listeria comprises a Nucleic Acid Sequence comprising one or More Open Reading Frames that encode one or more peptides comprising one or more neoep\u00edtopos,The process comprises: (a) obtain and identify such a Nucleic Acid Sequence encoding one or more peptides comprising one or more neoep\u00edtopos with disease in a sample of a subject who has a disease or condition (b) stably transfect Listeria attenuated strain AN EXPRESSION vector comprising the Nucleic Acid Sequence encoding the one or more p\u00e9ptidYou may include one or more neoep\u00edtopos (c) to obtain clones of Listeria expressing such one or more peptides that may include one or more neoep\u00edtopos (d) to expand such clones of Listeria to a Predetermined Scale (e) to purify the Listeria Expanded clones (f) to replace the Middle C Growth with Damper formulation (g) to gather such clones of Listeria dilute such clones (H)Listeria collected to obtain a Solution having a Predetermined Concentration and (i) Dispense the solution of Listeria clones collected in Single Dose containers for subsequent Storage and Administration to a subject, where the steps (d) - (i) was carried out in a s Cell growth System of Single Use completely closed.Claim 48: a tangential flow Filtration Device comprising: a bag of holding,Whose Bag includes: a Recirculation retained an entry exit Recirculation and an entry of diafiltration permeate Bag a filter and a circulation Pump where a First conduit defines a First Fluid path from the output to the Recirculation Entry of Recirculation, and where the first Fluid conduit connects the Bag Held,The circulation pump and the filter, so that the circulation Pump is configured to pump a mixture from the bag and held until the filter
