BEEVER, DAVID ELLISON,HALL, ANTHONY CHARLES,KEENAN, GERARD,MCCURDY, JOHN JOSEPH
申请号：
NZ58930309
公开号：
NZ589303A
申请日:
2009.05.27
申请国别(地区):
NZ
年份:
2012
代理人:
摘要：
A method for efficiently amplifying abnormal prion protein (PrPSc) derived from bovine spongiform encephalopathy (BSE). Based on protein misfolding cyclic amplification (PMCA). Normal prion protein (PrPC) is used as a source and PrPSc derived from BSE is used as a seed. The method comprises stirring, mixing, incubating, and sonicating both the PrPC and the PrPSc repeatedly in the presence of a polysaccharide sulphate. In particular the polysaccharide sulphate can be dextran sulphate or pentosan sulphate, wherein the dextran sulphate can be dextran sulphate sodium or dextran sulphate potassium.
