An aqueous composition having increased protein stability is obtained by: a. determining a pH at which the protein has stability at the desired temperature； b. adding to the composition at least one displacement buffer wherein the displacement buffer has a pKa that is at least 1 unit greater or less than the pH of step (a)； and c. adjusting the pH of the composition to the pH of step (a)； wherein the aqueous composition does not comprise a conventional buffer at a concentration greater than about 2 mM and wherein the conventional buffer has a pKa that is within 1 unit of the pH of step (a).
