The present disclosure relates to production of muscle cell precursors. In particular, the present disclosure relates to an in vitro method of producing skeletal muscle precursor cells from pluripotent stem cells. The present disclosure also further relates to the production of skeletal muscle cells (myoblasts and myocytes) from differentiated stem cells. The present disclosure also relates to the use of these cells in various research applications as well as their use in the treatment of skeletal muscle diseases or disorders.
