The present application relates to a method for controlling a size of lactic acid bacteria, wherein the method comprises passing a culture medium of lactic acid bacteria through a membrane filter. The size of the lactic acid bacteria is controlled by shear force applied to the lactic acid bacteria culture medium, wherein the shear force is controlled by a speed at which the lactic acid bacteria culture medium passes through the membrane filter. The size of the lactic acid bacteria can be easily controlled.본원은, 유산균 배양액을 막 필터에 통과시키는 것을 포함하는 유산균의 크기 조절방법에 관한 것이다.