CHILDREN'S HOSPITAL MEDICAL CENTER;LE PENDU, JACQUES
发明人:
JIANG, XI,LE PENDU, JACQUES
申请号：
CA2487846
公开号：
CA2487846C
申请日:
2003.06.02
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
A method for detecting a Norwalk-Like Virus (NLV) in a biological sample,comprising the steps of: obtaining a biological sample suspected of containinga NLV； contacting the biological sample with at least one human histo-bloodgroup antigen to allow formation of a complex of the NLV with the antigen； anddetecting the antigen NLV complex. The antigen-NLV complex can be detected bycontacting the NLV-antigen complex with a NLV antibody that binds at anepitope of the NLV, and detecting the NLV antibody. The invention alsoincludes a method for identifying compounds, and the compounds, that caninhibit the binding between a Norwalk-Like Virus (NLV) and a histo-blood groupantigen. The method includes the steps of contacting the NLV target with acompound； subsequently contacting the NLV with a standard compound that isknown to be bound at a determinant binding site of the NLV； and determiningwhether the binding of the standard compound is decreased in the presence ofthe test compound, the decrease in binding being an indication that the testcompound inhibits the binding activity of the NLV with the standard compound.In preferred embodiments, the standard compound is a histo-blood group antigen.
