Provided is a use of tobacco gene NtTCTP for resisting potato virus Y in plants (PVY). The tobacco gene has a full length of 507 bp and encodes a protein containing 168 amino acids, the activity of which is closely related to the PVY-resistance of tobacco. When the gene is silenced in tobacco, tobacco shows stronger resistance to PVY and can reach immune levels and when the gene is overexpressed in the tobacco and tobacco becomes more sensitive to PVY The gene can be used in the breeding of PVY-resistant plants such as tobacco.
