The invention relates to a cover for mushroom culture bottle and space bag cultivation, which comprises an upper cover, a base, a plug seat and a ventilating film. The upper cover is closed on the base, the ventilating film is formed and fixed on the base, and the large hole of the base is sealed. The plug seat is set on the content space of the base. By the composition of the above elements, the mushroom culture bottle and the The space bag can be fully imported into the automatic filling operation, which can effectively reduce the production cost and meet the environmental protection requirements.本創作係有關於一種菇類菌種瓶及太空包栽培用的封蓋,包含一上蓋、一底座、一塞座及一透氣薄膜,其中該上蓋蓋合於該底座上方,該透氣薄膜成型固設於該底座,並封合該底座的大通孔,該塞座套設於該底座的內容空間,藉由上述元件之組成,使菇類菌種瓶及太空包可完全導入自動化裝填作業,有效降低生產成本,又符合環保者。