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Transcription Factors that regulate the biosynthesis of nicotine in Tobacco
专利权人:
NORTH CAROLINA STATE UNIVERSITY
发明人:
RONGDA QU,BINGWU WANG
申请号:
ARP140100659
公开号:
AR094945A1
申请日:
2014.02.28
申请国别(地区):
AR
年份:
2015
代理人:
摘要:
1. Claim 1: a single nucleic acid molecule, including a sequence selected from a set of nucleosides, including: (a) nucleic acid sequences of SEC sections 1, 3, 5 and 7; (b) Nucleoside sequence, which is at least 95% the same as the above nucleoside sequence; (c) nucleoside sequence, which encodes a transcription factor, regulates nicotine biosynthesis according to ID No.2, ID No.4, ID No.6 and ID No.8; (d) sequence Nucleosides different from nucleoside sequence (a)(b) Or (c) due to the degradation of the genetic code; (E) a nucleoside sequence, mating under strict denuclearization conditions related to the nucleoside sequence;(b)(c) Or above (d) or its complement; and (f) any combination of above nucleoside sequences (a) - (E). Requirement 2: a nucleic acid structure, including the meaning of 5-3, 1. An initiator that can operate in the nucleic acid plant and molecular cells of claim 1, moves down from the initiator and has an operational connection with the initiator.Any nucleic acid required in Items 2 to 5 or carrier required in Item 6. Requirement 8: a transgenic plant including the plant cells required in Item 7. D. Claim 10: plant seeds or descendants of plants in claim 8 or claim 9The seed contains the nucleic acid structure of any 2-5 element in its genome. Claim 13: tobacco products of claim 12, wherein tobacco products are selected from one cigarette, cigarette, cigar, pipe, gum, leaf tobacco, chopped tobacco, smoked tobacco and any combination of tobacco. Requirement 16: a method to module al alkali content in a plant,2. It includes inputting the nucleic acid accumulation in any one of the two to five requirements into a cell of the plant to produce a cell of a transgenic plant, including the nucleic acid accumulation in any one of the two to five requirements; And regenerate the cell from the transgenic plant to produce a transgenic plant including the nucleic acid structure,Therefore, the production of nicotine was adjusted in the transgenic tobacco pl
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