The present disclosure provides a bacterium-containing hydrogel including: hydrogel, bacteria, and metabolites. The hydrogel including a plurality of polymer chains crosslinked with each other, each polymer chain including at least one polyethylene glycol chain. The bacteria are in the hydrogel, and the bacteria use the polymer chains as carbon source to ferment. The metabolites are produced during the fermentation of the polymer chains, and the metabolites distribute in the hydrogel. The present disclosure also provides a method for making bacterium-containing hydrogel.本揭示內容提供了一種包含細菌的水凝膠,包含:水凝膠,細菌,和代謝物。水凝膠包含互相交聯的多個高分子鏈,各高分子鏈包含至少一聚乙二醇鏈。細菌位於水凝膠內,且細菌以該高分子鏈為碳源發酵。代謝物為由細菌以該高分子鏈為碳源發酵時產生,並分布於該膠體中。本揭示內容亦提供一種包含細菌的水凝膠之製備方法。