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INDUCTION IN VITRO DE L'EXPANSION ET DE LA DÉRIVATION DE CELLULES SOUCHES ADULTES
专利权人:
CHANG-LIN, Samantha;LIN, Shi-Lung;CHANG, Donald
发明人:
LIN, Shi-Lung,CHANG-LIN, Samantha,CHANG, Donald
申请号:
USUS2018/051658
公开号:
WO2020/009714A1
申请日:
2018.09.19
申请国别(地区):
US
年份:
2020
代理人:
摘要:
This invention generally relates to a composition and its method of use for inducing adult stem cell (ASC) expansion and/or derivation in vitro, using miR-302-like pre-miRNAs, shRNAs and/or siRNAs, all of which contain a shared sequence of 5'-UAAGUGCUUC CAUGUUU-3' in the 5'-end, and further in conjunction with the use of some wound-healing-related defined factors, including but not limited to basic fibroblast growth factor (bFGF)/fibroblast growth factor 2 (FGF-2), leukemia inhibitory factor (LIF), insulin-like growth factor (IGF), Epidermal growth factor (EGF), platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), transforming growth factor (TGF), tumor necrosis factor (TNF), stem cell factor (SCF), homeobox proteins (HOX), Notch, GSK, Wnt/beta-Catenin signals, interleukins, and/or bone morphogenetic proteins (BMPs). The principle of the present invention is related to a novel mechanism of inducible symmetric ASC division recently found in a skin wound healing model in vivo. The resulting amplified ASCs are useful for treating a variety of human aging- and cell dysfunction-associated disorders, including but not limited to Alzheimer's disease, Parkinson's disease, motor neuron disease, stroke, diabetes, osteoporosis, myocardial infraction, hemophilia, anemia, AIDS, leukemia, lymphoma and many kinds of cancers as well as aging.La présente invention concerne de manière générale une composition et sa méthode d'utilisation pour induire l'expansion et/ou la dérivation de cellules souches adultes (ASC) in vitro, en utilisant des pré-ARNmi de type miR-302, des ARNsh et/ou des ARNsi, ceux-ci contenant tous une séquence partagée 5'-UAAGUGCUUC CAUGUUU-3' à l'extrémité 5', et de plus conjointement avec l'utilisation de certains facteurs définis liés à la cicatrisation des plaies, comprenant, mais sans y être limités, le facteur de croissance des fibroblastes basique (bFGF) / facteur de croissance des fibroblastes 2 (FGF-2), le facteur inhibiteur de
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