In one of the important aspect of the invention high performance thin layer chromatography (HPTLC) method simultaneous determination of combined dosage form of Salbutamol and Beclomethasone in Rota caps, the chromatograms were developed using a mobile phase of Toluene: Ethanol: Triethyl amine (6:2:1 v/v).The method uses aluminum plates coated with silica gel 60 F254 as stationary phase. Densitometry evaluation of the separated bands was performed at 270 nm.The Revalues for Salbutamol and Beclomethasone were 0.38 and 0.82 respectively. The linearity of Salbutamol and Beclomethasone is 1600 to 9600 ng/spot and 400 to 2400 ng/spot respectively. Recovery of Salbutamol and Beclomethasone is in between 100.02 to 101.99. Precision, Robustness and Ruggedness are shown in validation section. The proposed method HPTLC method is simple, economic, accurate & reproducible & can be used in routine analysis for simultaneous determination of Salbutamol and Beclomethasone in combined dosage form.
