Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
发明人:
申请号：
EP13004943.0
公开号：
EP2862874A1
申请日:
2013.10.15
申请国别(地区):
EP
年份:
2015
代理人:
摘要：
The invention relates to a method for producing polyclonal antibodies against an antigenic target protein which comprises inducing an immune response in a host by applying an immunogenic composition comprising membrane vesicles incorporating said antigenic target protein to said host and obtaining antibodies against said target protein from the host's serum.A more specific embodiment of this method comprises at least the following steps:a) synthesizing the target protein my means of an in vitro translation reaction in a reaction medium comprising a nucleic acid template coding for the target protein and a cell lysate which contains membrane vesicles；b) separating membrane vesicles comprising the synthesized target protein from the medium；c) providing the membrane vesicles comprising the synthesized target protein in a physiologically compatible medium；d) applying the membrane vesicles of step c) to a host；e) testing a serum sample of the host for a specific immune response against said target protein； andf) obtaining specific antibodies against said target protein from the serum of a host exhibiting a specific immune response against said target protein.
