A surgical marking pen may include a pen body, a first chamber within the pen body containing at least one powdered dye, a second chamber within the pen body and separated from the first chamber, and a tip in fluid communication with the first chamber or the second chamber. The second container contains a solvent, and the first chamber and the second chamber are selectively placeable into fluid communication with one another. The at least one powdered dye and the solvent, when mixed, form a dye solution which is disposable upon a skin or tissue surface and is able to be visualized simultaneously by a user in ambient light and with a fluorescence imager.
