The present invention relates to a vaccine containing immobilized virus particles, wherein the sum total of exothermic reactions in three rabbits in an exothermic test on the immobilized virus particles is less than 80% relative to the sum total of exothermic reactions in three rabbits in the exothermic test on virus particles from which the immobilized virus particles are originated or inactivated virus particles which correspond to the immobilized virus particles.本發明是關於一種疫苗,其包含固定化病毒粒子,其中,在放熱試驗中,以成為固定化病毒粒子的起源之病毒粒子或對應的去活化病毒粒子於3隻兔子中的放熱反應之和為基準計,固定化病毒粒子於3隻兔子中的放熱反應之和未達80%。