The invention relates to a two-piece cannula. The two-piece cannula comprises an outer cannula member that is engageable with an inner cannula member to define a continuous passage when the two cannula members are coupled together. When inserted in body tissue, the continuous passage can receive a surgical instrument, such as an arthroscope, for medically analyzing and/or treating a region of interest located beneath the body tissue. The outer cannula member and the inner cannula member comprise an outer portion and an inner flange, respectively, for sealing and securing the body tissue between the outer portion and the inner flange. In a further embodiment, the invention relates to kit comprising a two-piece cannula and an inserter for inserting the two-piece cannula into body tissue. In yet a further embodiment of the invention, a method is provided for inserting the two-piece cannula into body tissue.