the present invention is a method of \u043f\u0435\u0440\u0438\u043f\u043b\u0430\u0437\u043c\u0430\u0442\u0438\u0447\u0435\u0441\u043a\u043e\u0433\u043e \u044d\u043a\u0441\u043f\u0440\u0435\u0441\u0441\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f bacterial \u0430\u043d\u0430\u0442\u043e\u043a\u0441\u0438\u043d\u0430, comprising the steps: (a) the cultivation of a culture of \u0433\u0440\u0430\u043c\u043e\u0442\u0440 \u0438\u0446\u0430\u0442\u0435\u043b\u044c\u043d\u043e\u0439 cells - master in \u0444\u0435\u0440\u043c\u0435\u043d\u0442\u0430\u0446\u0438\u043e\u043d\u043d\u043e\u0439 environment where the cage is host to transform \u043f\u043e\u043b\u0438\u043d\u0443\u043a\u043b\u0435\u043e\u0442\u0438\u0434\u043e\u043c,which encodes bacterial toxoid and \u043f\u0435\u0440\u0438\u043f\u043b\u0430\u0437\u043c\u0430\u0442\u0438\u0447\u0435\u0441\u043a\u0443\u044e signaling sequence or the \u0433\u0440\u0430\u043c\u043e\u0442\u0440\u0438\u0446\u0430\u0442\u0435\u043b\u044c\u043d\u043e\u0439 host cells where the cell -. host ina transform \u043f\u043e\u043b\u0438\u043d\u0443\u043a\u043b\u0435\u043e\u0442\u0438\u0434\u043e\u043c, which encodes bacterial toxoid and \u043f\u0435\u0440\u0438\u043f\u043b\u0430\u0437\u043c\u0430\u0442\u0438\u0447\u0435\u0441\u043a\u0443\u044e signaling sequence, and where the pope anacletus shaped gram negative this is the master contains bacterial toxoid.\u044d\u043a\u0441\u043f\u0440\u0435\u0441\u0441\u0438\u0440\u043e\u0432\u0430\u043d\u043d\u044b\u0439 in \u043f\u0435\u0440\u0438\u043f\u043b\u0430\u0437\u043c\u0443 a1) \u0438\u043d\u0434\u0443\u0446\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f expression bacterial \u0430\u043d\u0430\u0442\u043e\u043a\u0441\u0438\u043d\u0430 b) the maturation of cell - host, where the maturation stage includes: (1) \u043f\u043e\u0434\u0432\u0435\u0440\u0433\u0430\u043d s cells - master ph shock (2) \u0438\u043d\u043a\u0443\u0431\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u0435 host cells without the add
