PURPOSE:To obtain in place of chemical synthetic process, the title steroid having plant growth-regulating effect, useful as a drug for the medical industry, by culture of the crown gall cells in Dicotyledoneae followed by isolation and purification from the resulting cultured product. CONSTITUTION:The crown gall cells in Dicotyledoneae is put to culture, and the objective steroid is isolated from the resulting cultured product. It is suggested that said be produced by infecting the seedlings of Catharanthus roseus L. with plant oncogenic bacteria, taking the tumor site on an artificial culture medium, carrying out sterilizing treatment and growing through liquid culture.
