As for this invention Bacillus barbaricus of oceanic origin SCSIO 02429 CCTCC NO: It regards M 2010213. In addition, the feature fermenting Bacillus barbaricus SCSIO 02429 in addition to the enzyme production fermentation induction nutrient medium, as for this invention in regard to the manufacturing method of ikaorigopepuchido, for hydrolysis roughly after obtaining the enzyme solution, crushing the squid internal organs in slurry condition and doing enzyme disassembly obtaining crude protein where the Glico sill side chain is destroyed enzyme disassembly ones in addition to the enzyme solution roughly, advancing enzyme disassembly furthermore including the bromelain, the quiet position doing enzyme disassembly ones which are obtained, making the oil stratum and water layer separate, removing the oil stratum and making dry you obtain ikaorigopepuchido. As for yield of ikaorigopepuchido of this invention 40 - 46%, as for yield of amino acid it reaches to 16 - 22%. There is the action which reduces the seed and seedling mortality rate of the oceanic cultivation animal to this ikaorigopepuchido, raises weight increasing rate, such as fish meal in the fodder because it is possible, to substitute to former protein source, it can use as functional protein source and the additive etc of oceanic cultivation formula feed.< Selective figure >It is not本発明は海洋由来のBacillus barbaricus SCSIO 02429 CCTCC NO:M 2010213に関する。また、本発明はイカオリゴペプチドの調製方法に関し、その特徴はBacillus barbaricus SCSIO 02429を酵素生産発酵誘導培地に加えて発酵させ、加水分解用粗酵素溶液を得た上で、イカ内臓をスラリー状に破砕して粗酵素溶液に加え、酵素分解を行ってグリコシル側鎖が破壊された粗タンパク質酵素分解物を得て、さらにブロメラインを加えて酵素分解を進め、得られた酵素分解物を静置して油層と水層に分離させ、油層を除去して乾燥させてイカオリゴペプチドを得る。本発明のイカオリゴペプチドの収率は40~46%、アミノ酸の収率は16~22%に達する。このイカオリゴペプチドには海洋養殖動物の種苗死亡率を下げ、体重増加率を高める作用があり、飼料中の魚粉など従来のタンパク質源に代わることができるため、海洋養殖配合飼料の機能性タンパク源、添加物などとして使用できる。【選択図】なし
