A method of delivering a therapeutic substance for treatment to a region of the body through vascular isolation and manipulation of fluid flux into and from the region of the body including the steps of: restricting vascular inflow to the region of the body； washing out oncotically active plasma proteins from the region of the body by increasing the outward oncotic pressure gradient from the region of the body； inducing ischemia in the region of the body； controlling the pressure and fluid flow of the main blood vessels to and from the region of the body； providing the therapeutic substance to the region of the body when the fluid flow to the region of the body is controlled.
