A guide for anatomical mapping of a face includes two flexible strips arranged at right angles with one of the strips slidable in its lengthwise dimension relative to the other strip. The other strip is provided with an arcuate end edge and a window predetermined based on facial anatomical features. The width and intersection of the strips are predetermined based on facial anatomical features. The guide is arranged in various positions on the face of a subject and in each position markings are made on the face of the subject along edges and through the window of the guide. This facilitates, for example, locating safe and effective injection sites for filler and the like.