Fyodor Urnov,Michael C. Holmes,Jeffrey C. Miller,Carl O. Pabo
申请号：
US13837027
公开号：
US09289451B2
申请日:
2013.03.15
申请国别(地区):
US
年份:
2016
代理人:
摘要：
Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.
